Corneal Abrasion Diagnosis with Fluorescein Dye
Corneal Abrasion Diagnosis with Fluorescein Dye A corneal abrasion, commonly known as a scratched cornea, is a painful injury that involves a superficial scratch or scrape on the cornea’s surface—the clear, dome-shaped front part of the eye. Proper diagnosis is crucial to prevent complications such as infection, scarring, or vision loss. One of the most effective and widely used methods for diagnosing corneal abrasions is the application of fluorescein dye combined with slit-lamp examination.
Fluorescein dye is a yellow-orange dye that is safe for ocular use. During the diagnostic process, the patient’s eyelids are gently separated, and a small amount of the dye is instilled into the conjunctival sac—either via a sterile strip soaked in fluorescein or through a drop of solution. The patient is then asked to blink several times to distribute the dye evenly across the corneal surface. After this, the eye is examined under a slit lamp with a cobalt blue filter, which causes the dye-stained areas to fluoresce bright green.
The fluorescein dye adheres specifically to areas where the corneal epithelium has been compromised, highlighting any abrasions or irregularities in the surface layer. The bright fluorescence makes even tiny or superficial scratches visible, which may not be apparent to the naked eye. The pattern, size, and location of the abrasion are carefully documented to guide treatment. Because fluorescein is highly sensitive to epithelial defects, it is an invaluable tool for rapid and accurate diagnosis.
In addition to identifying abrasions, fluorescein staining can help differentiate between superficial and deeper corneal injuries. For instance, deeper injuries may involve underlying stromal damage, which can be observed as staining extends beyond the superficial epithelium. This differentiation influences the management approach, as more extensive injuries may require specialized intervention to prevent complications like corneal ulceration.
While fluorescein dye is generally safe, some precautions are essential. Allergic reactions are rare but possible, especially in individuals with sensitivities to dyes or preservatives. Proper sterilization of equipment and the use of sterile techniques are critical to prevent infection. Furthermore, the procedure should be performed by trained healthcare professionals to ensure accurate application and interpretation, minimizing discomfort and potential harm.
Diagnosis using fluorescein dye is quick, minimally invasive, and highly reliable, making it the gold standard in assessing corneal abrasions. It allows clinicians to determine the precise extent of the injury, monitor healing, and decide on appropriate treatment, which may include antibiotic eye drops, analgesics, or protective eye patches. Follow-up examinations are often necessary to ensure complete healing and prevent secondary infections.
In summary, fluorescein dye application combined with slit-lamp examination provides an essential, effective technique for diagnosing corneal abrasions. Its ability to reveal minute epithelial defects ensures prompt and accurate diagnosis, ultimately safeguarding the patient’s vision and ocular health.









